Skip to main content
Figure 2 | Molecular Neurodegeneration

Figure 2

From: Apolipoprotein E4 (1–272) fragment is associated with mitochondrial proteins and affects mitochondrial function in neuronal cells

Figure 2

ApoE4 interacts with the subunits of mitochondrial respiratory complex III in Neuro2a cells. Neuro2a cells were cotransfected with mammalian expression plasmids encoding FLAG-apoE4(1–272) or FLAG-apoE4(1–299) and plasmids encoding mouse UQCRC2, human HA-UQCRC2, or human HA-cytochrome C1, all of which are candidate proteins suggested to be associated with apoE4 (Table 1). Twenty-four hours following the transfection, the cells were harvested and treated with 500 μl of Triton X-100 solubilization buffer to obtain cell lysate. The cell lysate was then incubated with anti-FLAG M2-agarose affinity resin, and the protein binding to the affinity column was eluted using FLAG peptide, and the eluted protein was then analyzed by western blotting with anti-UQCRC2 (mouse UQCRC2) antibody (A), anti-HA antibody (human UQCRC2) (B), or anti-HA antibody (human cytochrome C1) (C).

Back to article page