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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Apolipoprotein E4 (1–272) fragment is associated with mitochondrial proteins and affects mitochondrial function in neuronal cells

Figure 3

ApoE4 interacts with the subunits of mitochondrial respiratory complex IV in Neuro2a cells. Neuro2a cells were co-transfected with FLAG-apoE4 (1–272 or 1–299) plasmids and mammalian expression plasmids encoding the candidate apoE4-associated proteins. The cells were treated with 500 μl of Triton X-100 solubilization buffer and the cell lysate was incubated with anti-FLAG M2-agarose affinity resin. The immunoprecipitates were then analyzed by western blotting with an anti-COX IV 1 antibody (human COX IV 1).

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Molecular Neurodegeneration

ISSN: 1750-1326