Figure 5From: Apolipoprotein E4 (1–272) fragment is associated with mitochondrial proteins and affects mitochondrial function in neuronal cellsOverexpression of apoE4(1–272) results in the decreased level of complex III and IV activities. Enzymatic assays of respiratory chain complexes III (A) and IV (B) from Neuro2a cells overexpressing FLAG-apoE4(1–272 or 1–299) were determined as described in the Methods. The mitochondria levels in apoE4(1–272)- and apoE4(1–299)-overexpressing cells were determined by western blot analysis using the anti-UQCRC2 and the anti-cytochrome C1 antibodies (C). Data are the mean ± SEM of nine independent experiments. * P < 0.005, ** P < 0.0005 (t-distribution test).Back to article page