Detection of human and rodent IDE by 6A1 and 6H9 using western blotting and immunoprecipitation. A, Western blots of HeLa cell lysate (30 μg/lane) detected with individual anti-IDE mAbs (10 μg/mL) or, as a control, equivalent amounts of normal mouse IgG (NMI). For comparative purposes, the same cell lysate was probed with αIDE-1, a well-characterized rabbit polyclonal anti-IDE antibody (; generous gift of D. Selkoe, Harvard Medical School). B, Western blots of mouse liver extracts (30 μg/lane) detected with anti-IDE mAbs or NMI (10 μg/mL). Note that 6H9 labels multiple non-specific bands, but does not label rodent IDE per se (see text). C, Immunoprecipitation of human (upper panel) or rodent (lower panel) IDE by anti-IDE mAbs or NMI and detected by western blotting with αIDE-1. Note that rodent IDE was successfully immunoprecipitated by 6A1, exclusively.