Development of a sandwich ELISA for quantification of human IDE levels in brain extracts. A, Quantitative detection of recombinant human IDE by ELISA using 6H9 for capture and HRP-conjugated 6A1 for detection. B, Representative western blot showing variations in IDE protein levels in human cerebellar extracts detected with 2A1. C, Quantification of IDE by western blotting and 6H9/6A1 sandwich ELISA in 49 human cerebellar samples. For detailed methods, see Additional File 1.