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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Insulin-degrading enzyme is exported via an unconventional protein secretion pathway

Figure 2

A high-throughput-compatible fluorescence polarization-based assay for extracellular IDE activity detected by hydrolysis of FAβB. Time course of the hydrolysis of FAβB (0.5 μM) in HeLa cells transfected 2 days previously with human IDE cDNA (IDE), empty vector (Vect) or the latter treated with insulin (20 μM; Vect+Ins) to inhibit extracellular IDE activity and carried out in 384-well format. Data are mean ± SEM for 16 wells/condition.

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