Table 1 SORL1 PCR primers.
From: Expression of SORL1 and a novel SORL1 splice variant in normal and Alzheimers disease brain
Exons Amplified | Primer | Primer Sequence |
|---|---|---|
1-5 | Exon 1 Forward | GAGCTCTCTGCGAAGTCTGG |
| Â | Exon 5 Reverse | AAAAGGACTTGACATGTTCCTGA |
4-8 | Exon 4 Forward | GCCCAGTACCTCTGGATCAC |
| Â | Exon 8 Reverse | CTCACCAAGGTGTCACTGC |
7-11 | Exon 7 Forward | CATCTCTTGGGCAGTGAACA |
| Â | Exon 11 Reverse | CCACCTGGCTCCAGCACT |
10-15 | Exon 10 Forward | CCCATCCTGTCCAAGGAGT |
| Â | Exon 15 Reverse | AGTAGAACCCACAGGGCAAG |
14-18 | Exon 14 Forward | GAGTGTTTGCTGGGACACAA |
| Â | Exon 18 Reverse | TTTGAAGCCTGCATCTACCC |
17-21 | Exon 17 Forward | GTGGCCCTGGACTTTGACTA |
| Â | Exon 21 Reverse | CAGTGTTCTTCCCCTTGTAGAAA |
20-24 | Exon 20 Forward | TTACTGGACGGATGCCTACC |
| Â | Exon 24 Reverse | TTTCATCACTGTTGTCTCCACA |
23-29 | Exon 23 Forward | TGAGCGATGAGAGAAACTGC |
| Â | Exon 29 Reverse | TGGAAACCGAACTCATCACA |
28-32 | Exon 28 Forward | CGCACTTCATGGACTTTGTG |
| Â | Exon 32 Reverse | CCATCCTGGCAATCTTGGT |
31-35 | Exon 31 Forward | TGTCTGCCCAATTACTACCG |
| Â | Exon 35 Reverse | GTGCCTTGCTGAGACACTGA0 |
34-40 | Exon 34 Forward | ATGCCCTCTGCTTCTTGTGT |
| Â | Exon 40 Reverse | ATGCTTCCTCGGAAGTTCAA |
39-43 | Exon 39 Forward | ATCCCACCACCAGATATCCA |
| Â | Exon 43 Reverse | CCTGGTCAGGAGAGTCATACG |
42-46 | Exon 42 Forward | ACTCCACAACAAGACGGTCA |
| Â | Exon 46 Reverse | CAGCTCATCGTACAGCAGGA |
45-48 | Exon 45 Forward | CAGCACCTGATGCCTTAAAAA |
| Â | Exon 48 Reverse | CATATTGCAACTCAAAGTGCATC |
FL-SORL1 | Exon 2 Forward | GAGGCCCAAGAGCAGTGAT |
RT-PCR Primers | ||
Delta-2-SORL1 | Exon 1-3 Junction Forward | CAAGGTGTACGGACAGGTGTA |
Reverse Primer | Exon 4 Reverse | GCAGAAGTCAAACGTGATCC |