The CB2 antagonist, AM630, alters Akt and pAkt immunoreactivity in HT22 cells. (A), Treatment of HT22 cells with AM630 leads to an increase in nuclear Akt immunoreactivity (red arrow) and an increase in the nuclear over cytosolic Akt immunoreactivity ratio (graph) while the presence of PEA reduces this increase (graph). (B), Likewise AM630 increases nuclear pAkt immunoreactivity (red arrow) and increases the nuclear over cytosolic pAkt immunoreactivity ratio (graph) and while the inclusion of PEA reduces this effect (graph). Co-treatment of cells with both PEA and AM630 results in an increase in nuclear Akt (red arrow, graph). For the Akt study, n equals 50, 66, 52, 53, 62 and 65 cells for vehicle (PEA), PEA, vehicle (AM630), AM630, vehicle (PEA and AM630) and PEA and AM630 treatments, respectively. For the pAkt study, n equals 56, 75, 94, 78, 75 and 89 cells for vehicle (PEA), PEA, vehicle (AM630), AM630, vehicle (PEA and AM630) and PEA and AM-630 treatments, respectively. A P-value of ≤ 0.05, < 0.01 and ≤ 0.001 is indicated by *, ** and ***, respectively, as determined by a two-sample t-test.