Figure 5

PEA increases nuclear and cytosolic pERK1/2 and decreases nuclear and cytosolic phospho-p38 immunoreactivity in HT22 cells independent of CB2 activation. (A), A brief, 30 minute PEA treatment of HT22 cells has no effect on nuclear or cytosolic ERK1/2 immunoreactivity. (B), a 30 minute PEA treatment results in a transient increase in nuclear and cytosolic pERK/12 immunoreactivity. In the ERK1/2 immunofluorescence experiment, n equals 24 and 34 cells for vehicle and PEA treatments, respectively. In the pERK1/2 immunofluorescence experiment, n equals 46 and 81 cells for vehicle and PEA treatments, respectively. A P-value of ≤ 0.001 is indicated by *** as determined by a two-sample t-test. (C), Treatment of HT22 cells with PEA for 30 min. resulted in a dramatic and significant decrease in both nuclear and cytosolic phospho-p38 immunoreactivity. In this study, for the 30 minute treatment group, n equals 42 and 35 cells for vehicle and PEA treatments, respectively. For the 60 minute treatment group, n equals 50 and 41 cells for vehicle and PEA treatments, respectively. A P-value of ≤ 0.001 is indicated by *** as determined by a two-sample t-test.