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Table 5 Effects of High Fat Diet and NDEA Exposure on Expression of Pro-Ceramide Genes in Temporal Lobe

From: Nitrosamine exposure exacerbates high fat diet-mediated type 2 diabetes mellitus, non-alcoholic steatohepatitis, and neurodegeneration with cognitive impairment

 

LFD

LFD+NDEA

HFD

HFD+NDEA

F-Ratio

P-Value

CER1

6.710

6.055

6.794

7.381

  
 

0.651

0.685

0.984

0.996

  

CER2

19.230

4.858***

16.260

4.733**

18.780

< 0.0001

 

2.017

0.752

2.509

0.495

  

CER4

2.352

3.620*

2.770

4.608***

11.030

< 0.0001

 

0.139

0.147

0.249

0.495

  

CER5

18.370

4.898***

21.120

5.277***

18.540

< 0.0001

 

2.517

0.295

2.707

0.576

  

UGCG

13.670

29.960***

15.190

30.590***

15.330

< 0.0001

 

0.929

3.605

1.667

2.827

  

SMPD1

7.941

9.797

15.520***

9.490

6.919

0.001

 

0.766

1.435

1.841

0.859

  

SMPD3

4.405

28.910***

2.672

16.170**

21.170

< 0.0001

 

0.396

5.078

0.325

0.603

  

SPTLC1

0.066

0.038*

0.029**

0.042*

5.102

0.005

 

0.010

0.008

0.004

0.006

  

SPTLC2

1.728

1.107*

1.939

1.646

3.845

0.019

 

0.190

0.207

0.173

0.115

  
  1. Gene expression was measured by qRT-PCR with results normalized to 18S rRNA (see Methods). Values correspond to mRNA/18S × 10-6. Inter-group comparisons were made using ANOVA tests. For all measurements, df = 3/36. Significant differences relative to the LFD control group were demonstrated using the Dunnett's post-hoc multiple comparison test (*P < 0.05; **P < 0.01; ***P < 0.001).
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Molecular Neurodegeneration

ISSN: 1750-1326