Glut-5 levels in microglial CHME-5 cells. Figure 2A: Representative immunoblot showing the levels of Glut-5 protein (55 kDa, first row) in the cell lysates collected at the end of week 4 from the control and rotenone (5 nm) treated CHME-5 cells. β-actin (42 kDa, second row) was used as an internal loading control. Figure 2B: Histogram showing relative semi-quantitation of Glut-5 levels. Glut-5 levels were normalised against the β-actin levels in each well and expressed in densitometric units. P (p = 0.039) value indicates a significant difference in Glut-5 levels between control and rotenone treated groups. Each column represents the mean and standard deviation from three independent experiments. Figure 2C: Representative photomicrographs showing immunostaining of CR3/43 (HLA DR/DP/DQ) in control and rotenone treated CHME-5 cells at the end of week 4 of the treatment period. CR3/43 staining is dramatically increased in rotenone treated cells as visualised by the green fluorescence. Scale bar: 50 μm.