Skip to main content
Figure 5 | Molecular Neurodegeneration

Figure 5

From: Effects of chronic low dose rotenone treatment on human microglial cells

Figure 5

Superoxide dismutase (SOD) levels in both CHME-5 and SH-SY5Y cells. Figure 5A: Representative immunoblot and histogram showing levels of SOD1 in the two types of cells (CHME-5 and SH-SY5Y) collected from two treatment groups (DMSO control and rotenone treated). CHME-5 cells showed a significant (p < 0.05) two fold increase in SOD1 levels, whereas SH-SY5Y cells showed a more significant 2.4 fold increase (p < 0.01) in SOD1 levels after 4 weeks of rotenone treatment. The base levels of SOD1 appeared to be higher in SH-SY5Y cells compared to CHME-5 cells. Figure 5B: Representative immunoblot and histogram showing levels of SOD2 in the two types of cells (CHME-5 and SH-SY5Y) collected from two treatment groups (DMSO control and rotenone treated). CHME-5 cells did not show any change in SOD2 levels (p > 0.05), whereas SH-SY5Y cells showed a 1.5 fold increase in SOD2 levels after 4 weeks of rotenone treatment, although this increase was not significant (p > 0.05). The base levels of SOD2 appeared to be higher in SH-SY5Y cells compared to CHME-5 cells. 1: CHME-5 control; 2: CHME-5 rotenone; 3: SH-SY5Y control; 4: SH-SY5Y rotenone. Each column represents the mean and standard deviation from three independent experiments.

Back to article page