inhibitors did not affect the accumulation of PrP82-146 in synapses. The amount of PrP82-146 detected in synaptosomes isolated from cortical neurones incubated with a vehicle control (■), 1 μM AACOCF3 (□) or 1 μM MAFP (striped bars). Treated cortical neurones were incubated with 100 nM PrP82-146 for the time periods shown. Synaptosomes were isolated from neuronal cultures and the amount of PrP82-146 was measured by ELISA. Values shown are the mean average amount of PrP82-146 (nM) ± SD, n = 9.