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Table 1 PrP82-146 and Aβ1-42activated cPLA2within synapses.

From: Phospholipase A2inhibitors protect against prion and Aβ mediated synapse degeneration

Treatment Total cPLA2 protein
(units/mg protein)
Activated cPLA2
(units/mg protein)
None (units) 100 ± 31 100 ± 19
200 nM PrP82-146 106 ± 18 773 ± 51 *
200 nM PrP82-146scrambled 95 ± 22 111 ± 34
1 μM PLAP 119 ± 30 928 ± 84 *
100 nM Aβ 1-42 109 ± 21 655 ± 62 *
100 nM Aβ 42-1 92 ± 27 121 ± 34
  1. Primary cortical neurones were incubated with PrP, Aβ or PLAP peptides as shown for 1 hour. Synaptosomes were subsequently isolated from treated neurones and the amount of cPLA2 protein, and the amount of activated cPLA2 (phosphorylated at serine 505) were measured by ELISA. Values shown are the mean average amount of cPLA2 protein or activated cPLA2 (units/mg protein) ± SD, n = 12. * = amount of activated cPLA2 significantly greater than in control synaptosomes.