Effect of superoxide dismutase (SOD) on Aβ-induced ROS increase. ROS production was measured in rat cerebral microvascular endothelial and smooth muscle cells using the ROS sensitive dye MitoTracker Red CM-H2XRos (5 μmol/L). SOD was unable to attenuate Aβ1-40-induced reactive oxygen radical increase in either (A) endothelial (ECs) or (B) smooth muscle cells (SMCs). * denotes p > 0.05 from Control. N > three replicates with three cultures per replicate (n ≥ 9).