TY - JOUR AU - Fallini, Claudia AU - Bassell, Gary J. AU - Rossoll, Wilfried PY - 2010 DA - 2010/04/21 TI - High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function JO - Molecular Neurodegeneration SP - 17 VL - 5 IS - 1 AB - Cultured spinal motor neurons are a valuable tool to study basic mechanisms of development, axon growth and pathfinding, and, importantly, to analyze the pathomechanisms underlying motor neuron diseases. However, the application of this cell culture model is limited by the lack of efficient gene transfer techniques which are available for other neurons. To address this problem, we have established magnetofection as a novel method for the simple and efficient transfection of mouse embryonic motor neurons. This technique allows for the study of the effects of gene expression and silencing on the development and survival of motor neurons. SN - 1750-1326 UR - https://doi.org/10.1186/1750-1326-5-17 DO - 10.1186/1750-1326-5-17 ID - Fallini2010 ER -