Young and mature motor neuron cultures are efficiently transfected by magnetofection. 1 DIV (top), 2 DIV (middle), and 7 DIV (bottom) motor neurons were transfected with pmaxGFP. Twenty-four hours after transfection cells were fixed and stained with neurofilament antibody to identify neurons (NF, red). DAPI was used to stain nuclei (blue). GFP-positive cells (green) are visible in all tested conditions. Size bars: 100 μm.