Co-trafficking of Htt with BDNF 3'UTR mRNA in rat cortical neurons. (a) The BDNF mRNA reporter plasmid with short 3'UTR was co-transfected with plasmids expressing NLS-MS2-Venus and RFP-Htt480-17Q and examined by live-cell imaging. BDNF mRNA is shown in green (detected by NLS-MS2-Venus) and Htt in red. Htt co-localizing with BDNF mRNA is in yellow in merged images. (b) Four cropped images from a time-lapse series in (a) captured over 310.5 seconds. Htt is shown in grayscale and the merged image in yellow. Vertical white line is drawn through the four panels to highlight the movement of one mRNA granule (left of the white line) over time. The distance that the granule traveled was 1.76 μm. Scale bar: 10 μm.