Visualization of BDNF mRNA with Htt and mRNA-binding protein CPEB1 in vibratome sections of a rat brain cortex. (a) In merged panels, BDNF mRNA, Htt and CPEB1 were imaged in red, green and blue, respectively. The three left hand columns are presented in black and white. Reconstruction method: ImageJ, optical slice interval: 0.5 μm, stack size: 16.0 μm, rotation angle: 0°. N: Nucleus (b) Image stack in (a) was cropped for high-resolution 3D reconstruction. Yellow arrow indicates one co-localizing puncta of BDNF mRNA with Htt and CPEB1. Co-localized spots appear white in the merged panel. N: Nucleus. (c) High-resolution 3D presentation of (a) to demonstrate spatial relationship of Htt/BDNF mRNA/CPEB1. Rotation angle: 170°. Htt, BDNF mRNA and CPEB1 correspond to 1, 2, 3, respectively. Co-localization of Htt/mRNA (yellow), mRNA/CPEB1 (magenta), Htt/CPEB1 (cyan) and Htt/mRNA/CPEB1 (white) correspond to 4, 5, 6, 7, respectively.