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Table 2 Antibodies used in the study

From: Localization of BDNF mRNA with the Huntington's disease protein in rat brain

Antibody Immunogen Source Host species Isotype, Dilution used
anti-DIG digoxigenin conjugated with KLH Immunology Consultants Laboratory, CDIG-65A chicken polyclonal IgY, 1:500
anti-Htt human huntingtin protein a.a. 181-810 Millipore, MAB2166 mouse monoclonal, IgG1κ, clone 1HU-4C8, 1:500
anti-Ago2 human Ago2 peptide KLMRSASFNTDPYVRE Gift of Ramin Shiekhattar, The Wistar Institute rabbit polyclonal, 1:1000
anti-dynein HC rat dynein heavy chain a.a. 4320-4644 Santa Cruz Biotechnology, sc-9115 rabbit polyclonal, IgG, 1:300
anti-CPEB1 mouse CPEB1 peptide SMEGLRHHSPLMRNQKN Gift of David Wells, Yale University rabbit polyclonal, IgG, 1:1000
Alexa Fluor® 488 goat anti-mouse IgG mouse IgG (H + L) Invitrogen, A-11001 goat polyclonal, 1:500
Cy3 goat anti-mouse IgG mouse IgG (H + L) Jackson ImmunoResearch, 115-166-003 goat polyclonal, F(ab')2 fragment, 1:500
DyLight 549 goat anti-chicken IgY (IgG) chicken IgY (IgG) (H + L) Jackson ImmunoResearch, 103-505-155 goat polyclonal, 1:500
DyLight 649 goat anti-rabbit IgG rabbit IgG (H + L) Jackson ImmunoResearch, 111-495-144 goat polyclonal, 1:500
  1. Abbreviations: DIG, digoxigenin; KLH, keyhole limpet hemocyanin; Htt, huntingtin; Ago, Argonaute; CPEB, cytoplasmic polyadenylation element binding protein