Figure 4
No change in sodium channel total protein levels in BACE1-/- mouse brain. Whole brain or hippocampal homogenates from BACE1-/- mice and wild-type littermate controls were resolved by SDS-PAGE for Western analysis with antibodies against Nav1.2 and Nav1.6. Samples in A and B are whole brain homogenates from naïve animals; Samples in C and D are hippocampal homogenates from mice subjected to EEG recordings; Samples in E and F are whole brain homogenates from mice injected with 15 mg/kg (top) or 20 mg/kg (bottom) KA. Panels B, D and F are quantifications of sodium channel signals performed on the blots in panels A, C and E, respectively. No significant differences were detected in any comparison. All measures were normalized using β-tubulin signal. Error bars indicate SEM. In C, * indicates mice that exhibited spontaneous seizures and # indicates mice that exhibited abnormal background/spiking on EEG. In E, mice with seizure >4 (high seizure) and cell loss are indicated.