Figure 5
Na V 1.2 levels in mossy fibers do not correlate with seizure measures. Immunofluorescence labelling of Nav1.2 was performed on 30 μm brain sections, and confocal images of the CA3 region of the hippocampus were obtained. slu, stratum lucidum; DG, dentate gyrus. Sections in A are representative images from naïve mice, C, from mice subjected to EEG recordings, E, from mice treated with 15 mg/kg KA, and G, from mice treated with 20 mg/kg KA. Panels B, D, F and H are histograms representing quantifications of Nav1.2 fluorescence intensity in the stratum lucidum measured by ImageJ of all mice from the groups indicated. Mean fluorescence intensity within the stratum lucidum was measured and averaged over 2-4 sections/brain. In D, F, and H the subgroups with EEG abnormalities (EEG abn.) and severe seizures (high seiz.) did not have higher axonal Nav1.2 levels than the BACE1-/- group as a whole (middle bars). BACE1-/- mice with cell loss following 20 mg/kg KA treatment had significantly reduced Nav1.2 in the stratum lucidum. Error bars indicate SEM. n = 3 brains in B; n = 5, 13, 5 resp. in D; n = 3, 6, 3 resp. in F; n = 3, 6, 3 resp. in H. * p < 0.05. (no seiz., mice with no seizures; spon. seiz., mice with spontaneous seizures; seiz. ≤4, mice with seizures less than or equal to stage 4; seiz. >4, mice with seizures greater than stage 4)