Figure 2
PDGFRβ is not expressed in neurons or astrocytes in the adult 129S1/SvlmJ and F7 mouse brain. (a, b) Representative confocal imaging analysis of PDGFRβ immunodetection of perivascular pericytes (red), SMI-32 immunodetection of neuronal neurites (green), and lectin-positive brain capillaries (blue) in 6 month old 129S1/SvlmJ control and 6 month old F7 mouse layer II parietal cortex (a) and stratum radiatum of the CA1 hippocampal subfield (b). Scale bar 20 μm. (c, d) Representative confocal imaging analysis of PDGFRβ immunodetection of perivascular pericytes (red), NeuN-specific immunodetection of neurons (green), and lectin-positive brain capillaries (blue) in 6 month old 129S1/SvlmJ control and 6 month old F7 mouse layer III parietal cortex (c) and stratum pyramidale of the CA1 hippocampal subfield (d). Scale bar 20 μm. (e, f) Representative confocal imaging analysis of PDGFRβ immunodetection of perivascular pericytes (red), GFAP-specific immunodetection of astrocytes (green), and lectin-positive brain capillaries (blue) in 6 month old 129S1/SvlmJ control and 6 month old F7 mouse layer III parietal cortex (e) and stratum pyramidale of the CA1 hippocampal subfield (f). Scale bar 20 μm. (g) Representative confocal imaging analysis of PDGFRβ immunodetection of perivascular pericytes (red), NeuN-specific immunodetection of neurons (green), and lectin-positive brain capillaries (blue) in 6 month old 129S1/SvlmJ control and F7 mouse nucleus tractus solitarius in dorsal medulla oblongata. Scale bar 20 μm.