Skip to main content
Figure 1 | Molecular Neurodegeneration

Figure 1

From: Phosphorylation regulates proteasomal-mediated degradation and solubility of TAR DNA binding protein-43 C-terminal fragments

Figure 1

TDP-43 C-terminal fragments share similar pathological properties, such as phosphorylation and aggregation. M17 cells were transiently transfected to express GFP, GFP-TDP-43 or various GFP-TDP-43 C-terminal fragments for 2 days. (A) Western blotting of cell lysates using a phosphorylation-dependent (pS409/pS410) TDP-43 antibody (termed pTDP-43) or antibodies towards GFP, Hsp70, LC3B and GAPDH; the latter antibody was used to verify protein loading. (B) GFP fluorescence in cells expressing GFP, GFP-TDP-43 or GFP-tagged C-terminal fragments of TDP-43 was viewed by fluorescent confocal microscopy. Scale bar, 10 μM. Figures shown are representative of the results obtained from 2 independent experiments.

Back to article page