Figure 3
From: Increased amyloidogenic processing of transgenic human APP in X11-like deficient mouse brain
Human sAPP levels in APP23 mouse brain samples in the presence or absence of X11L. (A) Detection of total human sAPP, sAPPα and sAPPβswe along with human APP, X11L and α-tubulin in APP23 mouse brain tissue in the presence or absence of X11L. Membrane (P100 for APP) and cytoplasmic (S100 for sAPP, sAPPα, sAPPβswe, X11L and α-tubulin) fractions of brain regions composed of cerebral cortex, hippocampus and olfactory bulb from wild-type (WT), APP23 and APP23/X11L-Ko mice (5-6 months old) were analyzed for total human sAPP (sAPPα plus sAPPβswe by 10D1 antibody), human sAPPα (2B3 antibody), human sAPPβswe (6A1 antibody), human APP (10D1 antibody), X11L and α-tubulin by immunoblotting. (B) The densities of total human sAPP (left), sAPPα (middle) and sAPPβswe (right) bands were standardized to the density of α-tubulin, which was compared with the ratios for APP23 mice that were assigned a reference value of 1.0 (values represent the means ± S.E.). The data were analyzed by Student's t test (n = 10; **, p < 0.01).