Basal levels of HIF-1α RNA and protein are not significantly changed in Psen1-/- neurons. Basal HIF-1α RNA and protein were measured in neurons in the presence and absence of B27 supplement. In panel A, HIF-1α RNA was measured in neuronal cultures (n = 3/genotype) by qPCR. Samples were run in triplicate and normalized to the geometric means of Ppia and Gusb. In panel B, basal levels of HIF-1α protein were measured in neurons by Western blotting (n = 6 for cultures with B27 and n = 10 for B27 starved cultures). No statistically significant differences were found among the samples (unpaired t-tests). In panel C, representative Western blots are shown of cells cultured with B27 or without (-B27).