Figure 3
Mitochondrial potential loss produced by Aβ-AChE complexes. Hippocampal neurons were loaded with TMRM+ to measure changes in ΔΨmit. Fluorescence changes were recorded at 1 min intervals and are shown as ratio ΔF/Fo. (A) Representative photographs of neurons a time 0 (a) or after 30 min (b) of treatment with 5 μM Aβ-AChEf, in (c), the temporal quantification of the mitochondrial potential as indicated by the black bar on top of the graph. (B) Neurons treated with 5 μM Aβf (a) or 5 nM AChE (b). (C) Normalized fluorescence intensities at the end of each treatment are expressed over the control as follow: 5 μM Aβ; 5 μM Aβ-AChEf; 5 nM AChE; * and # p ≤ 0.001. (D) Neurons were loaded with Fluo-3 AM (black circle, 5 μM) and TMRM+ (white circle, 30 nM) to measure changes in the mitochondrial membrane potential (ΔΨmit). After a 5 min control period, 5 μM Aβ-AChEf was added (indicated by the black bar on top of the graph) followed by a wash with fresh recording media.