Lithium chloride, MK-801, and Wnt-7a prevent the calcium increase induced by the Aβ-AChE complexes. Hippocampal neurons were loaded with Fluo-3 AM and set for imaging in vivo experiments. Fluorescence changes were recorded at 1 min intervals and presented as the ratio ΔF/Fo. (A) Neurons were treated with 5 μM Aβ-AChEf alone (black circle) (a), or co-incubated with 10 mM LiCl (white square), or neurons were pre-incubated with Wnt-7a for 24 h before AChE-Aβ treatment (white circle) (b). Final normalized fluorescence intensities reached at the end of 1 h experiments are presented and expressed over the control situation using the following treatments: 5 μM Aβ-AChEf; 5 μM Aβ-AChEf with 10 mM LiCl (c); Wnt-7a pre-incubation for 24 h before beginning the in vivo experiments (d); *p = 0.001 compared to control; #p = 0.001 compared to Aβ-AChEf treatment. (B) Hippocampal neurons were treated with 5 μM Aβ-AChEf in the presence of 2 μM MK-801 during 1 h (a), and cytoplasmic calcium levels were detected by confocal microscopy. Quantification of three independent experiments after 1 h incubation (b); *p = 0.001 compared to Aβ-AChEf treatment.