Figure 4

zfPGRN-A knockdown results in disrupted development of multiple neural structures. Lateral views of whole-mount embryos probed with islet 1 at 36 hpf. (A), uninjected embryos (B) embryos injected with zfPGRN-A MO2 (C) embryos injected with 100 pg zfPGRN-A mRNA, or (D) embryos co-injected with zfPGRN-A MO2 + 100 pg zfPGRN-A. 96% (48/50) of uninjected embryos (A) and 90% (45/50) of embryos injected with 100 pg zfPGRN-A mRNA (B) showed islet1 expression in the developing hypothalamus, retinal ganglia, sensary ganglia and pharyngeal arches. 84%(31/37) of embryos injected with 10 ng of zfPGRN-A MO2 (C) showed disrupted development of multiple neural structures. Co-injection of embryos with 10 ng MO2 and 100 pg zfPGRN-A mRNA (D) resulted in the rescue of 80% (40/50) embryos displaying islet 1 positive neural populations. Scale Bar = 100 μm