Progranulin knockdown and over-expression validation: Average number of Truncated (A) and Branched (B) CaP MNs (n≥50/group). Values were expressed as mean ± standard error of the mean. Statistical analyses were done with one-way ANOVA followed by student-Newman-keuls Multiple Comparisons Test (p < 0.001-***, p < 0.01-**, p < 0.05-*). Error bars represent s.e.m. Abbreviations: WT, wild type; Control-MO, Control Morpholino; zfPGRN-A, zebrafish PGRN-A mRNA; hPGRN, human progranulin; MO1, Morpholino directed against the initiator codon ATG; MO2, Morpholino directed against the 5'UTR.