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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Systemic treatment with liver X receptor agonists raises apolipoprotein E, cholesterol, and amyloid-β peptides in the cerebral spinal fluid of rats

Figure 5

ABCA1 and apoE mRNA expression levels were upregulated in the mouse brain by LXR agonist treatment. ApoE and ABCA1 mRNA as well as protein expression were examined in the mouse brain by Western blot analysis and in situ hybridization following GW3965 treatment (50 mpk for 10 days). A: RIPA and DEA-soluble brain homogenates were prepared as described in Methods. Equal amounts of supernatant proteins were loaded per lane and actin or ApoJ was used as an additional loading control. Densitometric analysis of Western blots for ABCA1 (left panel) and apoE (right panel) levels in the brain of mice treated with vehicle or GW3965. * p < 0.05 compared to vehicle treated controls by ANOVA, N = 9 per group. B: Brain distribution of apoE mRNA by in situ hybridization showing predominant astrocytic expression and increased expression following treatment with GW3965. C: Marked increase of apoE mRNA was observed in the ependymal cells lining the lateral ventricle following LXR agonist treatment. D: In situ hybridization for ABCA1 mRNA also showing high up-regulation in the lateral ventricle by GW3965 (←).

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Molecular Neurodegeneration

ISSN: 1750-1326