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Figure 6 | Molecular Neurodegeneration

Figure 6

From: Systemic treatment with liver X receptor agonists raises apolipoprotein E, cholesterol, and amyloid-β peptides in the cerebral spinal fluid of rats

Figure 6

LXR agonist-stimulated cholesterol efflux is apoE- and ABCA1- dependent. The role of ABCA1 in the T1317-induced cholesterol efflux to exogenous apoE was examined in the CCF-STTG1 human astrocytoma cell line. A: HEK 293 cells were transiently transfected with human apoE3 cDNA (E3) or empty vector (V) and concentrated serum-free conditioned media was analysed on Western blotting for apoE content against human recombinant apoE3. B: Equal amounts of conditioned media protein containing approximately 50 μg/ml of HEK-apoE3 were added to CCF-STTG1 astrocytoma cells pre-loaded with cholesterol in serum-free media containing 5 μM T1317 or 0.05% DMSO (vehicle control). 48 h-conditioned media was then analyzed for total cholesterol and protein content while cell monolayers were lysed for Western blotting analysis of ABCA1 protein levels. Treatment with T1317 (5 μM) up-regulated the expression of ABCA1 and increased the release of cholesterol to HEK-apoE3, but not to vector-HEK media. C: Knock-down of ABCA1 with specific siRNA inhibited T1317-induced ABCA1 up-regulation and cholesterol release to HEK-apoE3 media. *p < 0.05 and **p < 0.01 by ANOVA.

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