Figure 2

α-Synuclein-induced cell death and parkin protection in gene transfer animal models. Silver staining in sections of rat striatum injected with A). lentiviral LacZ, B). lentiviral parkin and C). lentiviral α-Synuclein (arrows indicate silver-positive cells) and D). lentiviral parkin+α-Synuclein. Sections of rat striatum stained with TH antibody in lentiviral lacZ (Figure 2E), parkin (Figure 2F), α-Synuclein (Figure 2G) and parkin+α-Synuclein (Figure 2H) injected brains. Silver staining in 20 micron thick sections of rat SN from brains injected with I). lentiviral LacZ, J) parkin, K). α-Synuclein and L). parkin+α-Synuclein. TH staining of SN neurons from brains injected with M). lentiviral LacZ, N) parkin, O). α-Synuclein and P). parkin+α-Synuclein. Q). Histogram represents caspase-3 activity assay. Asterisk is significantly different to LacZ control, ANOVA, with Neumann Keuls multiple comparison N = 4, P < 0.05.