Figure 3
From: Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model
α-Synuclein-induced neuro-inflammation and parkin protection. Immunostaining with microglia IBA-1 antibody in 20 micron thick striatal sections of rat brains injected with A). lentiviral LacZ (Lv-LacZ), B). parkin (Lv-Par), C). α-Synuclein (Lv-Syn) and D). Lentiviral parkin+α-Synuclein (Lv-Par+Lv-Syn). Immunostaining with astrocytic GFAP in rat striatum injected with E). lentiviral LacZ, F). parkin, G). α-Synuclein and H). Lentiviral parkin+α-Synuclein I). Histograms represent stereological counting of IBA-1 and GFAP positive cells. J). Western blot of TNF-α and iNOS in striatal extracts analyzed on 10% NuPAGE SDS gel (Invitrogen) and I). Densitometric analysis of immunoblots, Asterisk is significantly different to LacZ control or as indicated, ANOVA, with Neumann Keuls multiple comparison N = 4, P < 0.05.