Figure 5
From: Parkinson-related parkin reduces α-Synuclein phosphorylation in a gene transfer model
Parkin expression prevents α-Synuclein phosphorylation and increases phosphatase activity. A). WB of fractionated α-Synuclein from α-Synuclein stably transfected MN9 D cells and infected with lentiviral parkin and B). WB analysis of striatal brain extracts using 4-12% NuPAGE SDS gel (Invitrogen) probed with various α-Synuclein antibodies and PLK2 antibody. C, D&E). Densitometry of blots expressed as % control. F). WB analysis of protein phosphatase-2A subunits from striatal brain extracts and. G). Densitometry of blots expressed as % control. H). WB of α-Synuclein stably transfected MN9 D cells infected with lentiviral parkin and treated with PP2A inhibitor okadaic acid, and I) WB of T240R mutant and PP2A subunits. J). Phosphatase activity assay. Asterisk is significantly different, ANOVA with Neumann Keuls multiple comparison N = 4 for animals, N = 6 for MN9 D cells, P < 0.05.