Substrate | % Activity Remaining |
---|
 | Bestatin | Puromycin |
o-phenanthroline
| PMSF |
---|
 | (2 mM) | (2 mM) | (2 mM) | (2 mM) |
---|
Alanine 4MNA | 0 | 0 | 0 | 79 |
Dynorphin A | 0 | 0 | 0 | 79 |
- PSAsf was preincubated with protease inhibitors at 2 mM on ice for 15 min. PSA activity was then assayed with alanine 4MNA, Dynorphin A. PSAsf incubated with buffer was taken as 100% to determine the remaining PSA activity of each treatment.