Figure 4From: NO signaling and S-nitrosylation regulate PTEN inhibition in neurodegenerationNO signals induce enhanced ubiquitination of PTEN, leading to protein degradation. (A) Treatments with SNOC, glutamate or Aβ peptides increase PTEN ubiquitination, as determined by IP-Western analysis. (B) Enhanced physical interaction between PTEN and NEDD4-1 upon various treatments at the conditions used in other experiments, as determined by co-immunoprecipitation/Western blot analysis. Figure is chosen as the representative of three independent experiments. (C) Downregulation of NEDD4-1 by siRNA ( 4 μg) prevents PTEN protein degradation upon SNOC treatment.Back to article page