Figure 2From: Axotomy-induced neurotrophic withdrawal causes the loss of phenotypic differentiation and downregulation of NGF signalling, but not death of septal cholinergic neuronsThe number of neurons that are positive only for p75 is increased in the ipsilateral side after axotomy. A, Confocal microscopy of double-immunofluorescence anti-ChAT/anti-p75 reveals an increase in the number of neurons that are positive only for p75 at different times after the axotomy. The superior panel is a panoramic view of the medial septum at 14 days after axotomy (scale bar: 1 mm). The central and inferior panels are magnification insets (scale bar: 200 μm) of neurons contralateral and ipsilateral to the lesioned side, showing p75-positive and ChAT-negative cells as indicated by the white arrows. B, The graph shows the percentage of total septal neurons expressing p75 that are p75-positive and ChAT-negative at 1, 3, 7 or 14 days after axotomy. Black bars represent the control side of the septum and white bars represent the side ipsilateral to the lesion. Asterisk indicates significance level p < 0.05.Back to article page