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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Abnormal iron metabolism in fibroblasts from a patient with the neurodegenerative disease hereditary ferritinopathy

Figure 4

Cellular iron levels. a) Fibroblasts were cultured under basal conditions (BC) and total iron content was analyzed by the ferrozine method. A statistically significant difference (*p < 0.05) in the levels of iron content (nmol Fe/mg protein) was observed in HF compared to non-HF fibroblasts. b) Analysis of the labile iron pool (LIP) in basal conditions and after treatment with 100 μM FAC for 72 h. The LIP was measured using the metal-sensitive fluorescence probe calcein. No statistically significant differences were observed under basal conditions. After FAC treatment, LIP levels increased significantly in both non-HF and HF fibroblasts. However, FAC-induced levels of LIP were significantly lower in HF compared to non-HF fibroblasts. *p < 0.05

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