Figure 1

3×Tg-AD mice have higher levels of TDP-43 and TDP-35. (A) Representative Western blots of proteins from the low salt fraction extracted from 2-month-old 3×Tg-AD and NonTg mice and probed with the indicated antibodies. A longer exposure time was necessary to see the less abundant low molecular weight fragments. (B) Quantitative analysis of the blots shows that at 2 months of age the levels of TDP-43 and TDP-35 were similar between 3×Tg-AD and NonTg mice (n = 6/genotype). (C) Representative Western blots of proteins from the low salt fraction extracted from 6-month-old 3×Tg-AD and NonTg mice. (D) Quantitative analysis of the blots shows that at 6 months of age the levels of TDP-43 and TDP-35 were significantly increased (p = 0.04 and p = 0.01, respectively) in the brains of the 3×Tg-AD mice compared to age- and gender-matched NonTg mice (n = 6/genotype). (E) Representative Western blots of proteins from the low salt fraction extracted from 12-month-old 3×Tg-AD and NonTg mice (n = 6/genotype). (F) Quantitative analysis of the blots shows that at 12 months of age the levels of TDP-43 and TDP-35 were similar between 3×Tg-AD and NonTg mice (n = 6/genotype). Abbreviation: exp = exposure. Data are presented as ± SEM and analyzed by t-test analysis.