Figure 3
Age-dependent changes in TDP-43 and TDP-35 cellular localization. (A) Representative Western blots of proteins from the cytosolic and nuclear fractions extracted from 2-month-old NonTg and 3×Tg-AD mice and probed with the a polyclonal anti-TDP antibody. A longer exposure was necessary to see the less abundant low molecular weight fragments. (B-C) Quantitative analysis of the blots shows that at 2 months of age the nuclear and cytosolic levels of TDP-43 and TDP-35 were not statistically significant between 3×Tg-AD and NonTg mice (n = 6/genotype). (D) Representative Western blots of proteins from the cytosolic and nuclear fractions extracted from 6-month-old NonTg and 3×Tg-AD mice and probed with a polyclonal anti-TDP antibody. (E-F) Quantitative analysis of the blots shows that at 6 months of age the nuclear levels of TDP-43 and TDP-35 were not statistically significant between 3×Tg-AD and NonTg mice (n = 6/genotype). In contrast, the cytosolic levels of TDP-43 and TDP-35 were significantly higher in the 3×Tg-AD mice compared to NonTg mice (p = 0.03 and p = 0.01, respectively). (G) Representative Western blots of proteins from the cytosolic and nuclear fractions extracted from 12-month-old NonTg and 3×Tg-AD mice and probed with a polyclonal anti-TDP antibody. (H-I) Quantitative analysis of the blots shows that at 12 months of age the nuclear and cytosolic levels of TDP-43 and TDP-35 were not statistically significant between 3×Tg-AD and NonTg mice (n = 6/genotype). Abbreviation: exp = exposure. Data are presented as ± SEM and analyzed by t-test analysis.