Figure 4
TDP-43 and TDP-35 levels do not correlate with tau phosphorylated at Thr181. (A) Representative microphotographs of 3×Tg-AD brain sections depicting hippocampal CA1 pyramidal neurons stained with the anti-tau antibody AT270, which recognize tau phosphorylated at Thr181 (n = 6/age group). (B) Representative Western blots of proteins extracted from 6- and 12-month old 3×Tg-AD mice probed with the AT270 antibody. β-actin is used as a loading control. (C) Quantitative analysis of the blots shows that the steady-state levels of AT270 were significantly higher in the brains of 12-month-old 3×Tg-AD mice compared to 6-month-old mice (n = 6/age group; p < 0.05). Data are presented as ± SEM and analyzed by t-test analysis. (D-E) Linear regression analysis indicated that the levels of AT270 in the brains of 6-month-old 3×Tg-AD mice did not correlate with the levels of TDP-43 (r2 = 0.09148, p = 0.56) and TDP-35 (r2 = 0.1491, p = 0.45). (F-G) Similarly, no correlation between AT270 and TDP-43 (r2 = 0.4644, p = 0.14) and TDP-35 (r2 = 0.4469, p = 0.15) levels was observed at 12 months of age.