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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Age-dependent changes in TDP-43 levels in a mouse model of Alzheimer disease are linked to Aβ oligomers accumulation

Figure 5

Aβ oligomers correlate with TDP-43 and TDP-35. (A-D) Representative microphotographs showing 3×Tg-AD hippocampal sections stained with the antibody M71/3, which is specific for Aβ oligomers. Panels C and D show higher magnification views of panels A and B, respectively (n = 6/age group). (E) Representative dot blots of protein extracted from 2-, 6-, and 12-month-old 3×Tg-AD mice and probed with the antibody A11, which is specific for Aβ oligomers. (F) Quantitative analysis of the dot blots shows that at 6 months of age, A11 levels were significantly higher compared to 2-month-old mice (p < 0.001) and 12-month-old mice (p < 0.05). (G-H) Linear regression analysis indicated that the levels of A11 in the brains of 6-month-old 3×Tg-AD mice significantly correlated with the levels of TDP-43 (r2 = 0.9330, p = 0.001) and TDP-35 (r2 = 0.6749, p = 0.045). (I) Similarly, the levels of A11 in the brains of 12-month-old 3×Tg-AD mice significantly correlated with TDP-43 (r2 = 0.7259, p = 0.031). (J) A strong trend was observed when analyzing A11 and TDP-35 levels in 12-month-old 3×Tg-AD mice (r2 = 0.6277, p = 0.06). The dotted lines in panels G-J represent 95% confidence intervals.

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