Figure 3
From: A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
Effect of N256S-kinesin-1A on the frequency and flux of neurofilament movement. Analysis of neurofilament movement through gaps in axons of cortical neurons expressing GFP-NFM alone (A & D; 51 movies), GFP-NFM plus N256S-kinesin-1A (B & E; 40 movies), or GFP-NFM plus exogenous wild type kinesin-1A (C & F; 50 movies). (A-C) Histograms of the frequencies of neurofilament movement, expressed as number of neurofilaments per hour. The filaments were classified as anterograde or retrograde based on their preferred direction of movement and an anterograde and retrograde frequency was calculated for each movie. (see Methods). (D-F) Histograms of the neurofilament fluxes, expressed as total distance moved by all the filaments per axon per hour in either the anterograde or retrograde direction. An anterograde and retrograde flux was calculated for each movie.