Figure 4
From: A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
Effect of N256S-kinesin-1A on the velocity, distance and duration of bouts of neurofilament movement. Analysis of the velocity, distance and duration of bouts of neurofilament movement in axons of cortical neurons expressing GFP-NFM alone (A, D & G; 246 anterograde bouts, 212 retrograde bouts), GFP-NFM plus N256S-kinesin-1A (B, E & H; 84 anterograde bouts, 91 retrograde bouts), or GFP-NFM plus exogenous wild type kinesin-1A (C, F & I; 254 anterograde bouts, 223 retrograde bouts). The anterograde and retrograde bouts are represented by the dark grey and light grey bars, respectively. The y-axis represents the percentage of bouts in the corresponding direction. Note: to avoid compressing the scale on the x-axis, the right-most bin represents an expanded bin size of 30-50 in graphs D-F, and 100-500 in graphs G-I.