Figure 7
From: A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport
Microtubules in cortical neuron axons are plus-end distal. Cortical neurons were transfected with YFP-EB1, which is a protein that binds to the growing plus ends of microtubules. Axons were imaged for 2 minutes at 2 second intervals in order to determine the direction of microtubule growth. (A) A typical kymograph showing a number of YFP-EB1 comets moving anterogradely. The horizontal dimension represents distance and the vertical dimension represents time. This kymograph was generated from the movie named Additional file 6 in the Supplementary Data. (B) Histogram of YFP-EB1 comet velocities (192 comets from 23 different neurons). Note that almost all the comets move anterogradely, confirming the plus-end distal orientation of the microtubules in these axons.