Figure 3From: ADAR2-dependent RNA editing of GluR2 is involved in thiamine deficiency-induced alteration of calcium dynamicsEffects of TD on the editing efficiency of GluR2 mRNA. A: Cortical neurons were treated with amprolium (Amp, 0 or 1 mmol/L) for 1 or 4 days (TD1 or TD4). Total RNA was isolated and PCR products of GluR2 were sequenced as described under the Materials and Methods. Sequence chromatograms of GluR2 transcripts were presented. Q/R indicates the glutamine/arginine editing position. The ratio of G (black) to A (green) reflects the editing efficiency. B: The Q/R site editing efficiency was calculated. The results were expressed as the mean ± SEM. *p <0.001. The experiments were replicated three times.Back to article page