Figure 4

The expression of ADAR2 in cultured cortical neurons. A: Cortical neurons were treated with amprolium (Amp, 0 or 1 mmol/L) for 1, 3 or 5 days. The expression of ADAR1 and ADAR2 was examined by immunoblotting analysis. The expression of α-tubulin served as a loading control. B: The relative amounts of ADAR1 or ADAR2 protein were measured microdensitometrically and normalized to the expression of α-tubulin. C: TD was induced in mice as described under the Materials and Methods. At specified times after TD, the brain was removed (n = 5 for each treatment group). The expression of ADAR1 and ADAR2 was determined by immunoblotting analysis. D: The relative amounts of ADAR1 and ADAR2 protein in the brain were measured microdensitometrically and normalized to the expression of α-tubulin. E: Cortical neurons were treated with amprolium (Amp, 0 or 1 mmol/L) for 1, 3 or 5 days. The relative mRNA levels of ADAR1 and ADAR2 were quantified with real time PCR as described under the Materials and Methods. F: TD was induced in mice as described above. At specified times after TD, the brain was removed (n = 5 for each group). The relative mRNA levels of ADAR1 and ADAR2 were quantified with real time PCR. The results were expressed as the mean ± SEM. *p < 0.05. The experiments were replicated three times.