Effects of TD on ADAR2 self-editing activity in cultured cortical neurons. A: Cortical neurons were treated with amprolium (Amp, 0 or 1 mmol/L) for 1 or 4 days (TD1 or TD4). The expression of ADAR2 mRNA was analyzed by RT-PCR as described under the Materials and Methods. The products of alternative splicing of ADAR2 mRNA (+ 47 nt) were examined. B: The self-editing efficiency of ADAR2 mRNA was quantified by calculating the percentage of alternative splicing products (+47 nt). The results were expressed as the mean ± SEM. *p < 0.05. The experiments were replicated three times.