The transfection of ADAR2 cDNA and shADAR2 RNA in cultured cortical neurons. A. Cortical neurons were transfected with empty vector pcDNA6, pcDNA6 carrying wild-type ADAR2 cDNA, scramble short hairpin RNA (shCon) and shRNA for ADAR2 (shADAR2) along with a YFP construct for two days as described under the Materials and Methods. The expression of ADAR2 was determined by immunoblotting analysis. B: The expression of ADAR2 was quantified microdensitometrically and normalized to the expression of α-tubulin. The results were expressed as the mean ± SEM of three independent assays. *p < 0.05.